A serotype-specific epitope of dengue virus 1 identified by phage displayed random peptide library

Epitope Screening by Use of a Random Peptide-Displayed Phage Library and Polyclonal Antibody-Coupled Liposomes

Yoichi KUMADA, Masao NOGAMI, Fida HASAN, Masaaki TERASHIMA and Shigeo KATOH Department of Bioscience and Biotechnology, Okayama University, 1-1, Tsushimanaka 3, Okayam-shi, Okayama 700-8530, Japan Graduate School of Science and Technology, Kobe University, 1-1, Rokkodaicho, Kobe-shi, Hyogo 657-8502, Japan Graduate school of Human Sciences, Kobe College, 4-1, Okadayama, Nishinomiya-shi, Hyogo 66...

Identification of a Conserved B-cell Epitope on Reticuloendotheliosis Virus Envelope Protein by Screening a Phage-displayed Random Peptide Library

BACKGROUND The gp90 protein of avian reticuloendotheliosis-associated virus (REV-A) is an important envelope glycoprotein, which is responsible for inducing protective antibody immune responses in animals. B-cell epitopes on the gp90 protein of REV have not been well studied and reported. METHODS AND RESULTS This study describes the identification of a linear B-cell epitope on the gp90 protei...

Phage-Displayed Random Peptide Library -Mediated Cytotoxicity Identified from a α A Peptidomimetic Antagonist of TNF-

Identification of epitopes in cucumber mosaic virus using a phage-displayed random peptide library.

Antigenic sites in the cucumber mosaic virus (CMV) coat protein (CP) have been identified using a polyclonal antiserum prepared against glutaraldehyde-fixed virions. Antibodies were used to screen a random peptide library of heptamers displayed on the surface of a bacteriophage. Eight of 36 (22%) sequenced phage clones had inserts resembling a putative virion surface domain of the CMV CP. This ...

Identification of a Conserved Linear B-Cell Epitope of Streptococcus dysgalactiae GapC Protein by Screening Phage-Displayed Random Peptide Library

The GapC of Streptococcus dysgalactiae (S. dysgalactiae) is a highly conserved surface protein that can induce protective humoral immune response in animals. However, B-cell epitopes on the S. dysgalactiae GapC have not been well identified. In this study, a monoclonal antibody (mAb5B7) against the GapC1-150 protein was prepared. After passive transfer, mAb5B7 could partially protect mice again...